NFIB antibody (AA 1-233)

Catalog No. ABIN7441289

This Rabbit Polyclonal antibody specifically detects NFIB in WB, IP, IHC and ICC. It exhibits reactivity toward Human.

Quick Overview for NFIB antibody (AA 1-233) (ABIN7441289)

Target: NFIB (Nuclear Factor I/B (NFIB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFIB antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-NFIB Antibody

Binding Specificity: AA 1-233

Purpose: Polyclonal Antibody to Nuclear Factor I/B (NFIB)

Specificity: The antibody is a rabbit polyclonal antibody raised against NFIB. It has been selected for its ability to recognize NFIB in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Nuclear Factor I/B (NFIB) corresdonding to Met1~Gln233 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:250-1000Immunohistochemistry: 5-20 μg/mL,1:25-100Immunocytochemistry: 5-20 μg/mL,1:25-100Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for NFIB

Target: NFIB (Nuclear Factor I/B (NFIB))

Alternative Name: NFIB

Background: NFI-RED, NFIB2, NFIB3, CCAAT-box-binding transcription factor, TGGCA-binding protein

UniProt: O00712