Notch1 antibody (AA 1919-2079)

Catalog No. ABIN7445663

This Rabbit Polyclonal antibody specifically detects Notch1 in WB, IHC, ICC and IP. It exhibits reactivity toward Mouse.

Quick Overview for Notch1 antibody (AA 1919-2079) (ABIN7445663)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Notch1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-Notch1 Antibody

Binding Specificity: AA 1919-2079

Purpose: Polyclonal Antibody to Translocation Associated Notch Homolog 1 (TAN1)

Specificity: The antibody is a rabbit polyclonal antibody raised against TAN1. It has been selected for its ability to recognize TAN1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Translocation Associated Notch Homolog 1 (TAN1) corresdonding to Glu1919~Ile2079 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Alternative Name: TAN1

Background: NOTCH1, hN1, NEXT, NICD, Translocation-associated notch protein TAN-1

UniProt: Q01705

Pathways: Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development