PHPT1 antibody (AA 1-124)

Catalog No. ABIN7437653

The Rabbit Polyclonal anti-PHPT1 antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect PHPT1 in samples from Mouse.

Quick Overview for PHPT1 antibody (AA 1-124) (ABIN7437653)

Target: PHPT1 (phosphohistidine Phosphatase 1 (PHPT1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHPT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-PHPT1 Antibody

Binding Specificity: AA 1-124

Purpose: Polyclonal Antibody to Phosphohistidine Phosphatase 1 (PHPT1)

Specificity: The antibody is a rabbit polyclonal antibody raised against PHPT1. It has been selected for its ability to recognize PHPT1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Phosphohistidine Phosphatase 1 (PHPT1) corresdonding to Met1~Tyr124 (Accession # Q9DAK9)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PHPT1

Target: PHPT1 (phosphohistidine Phosphatase 1 (PHPT1))

Alternative Name: PHPT1

Background: PHP14, Phosphohistidine Phosphatase 14 kDa, Sex-Regulated Protein Janus-a, Protein janus-A homolog

UniProt: Q9DAK9

Pathways: Positive Regulation of Peptide Hormone Secretion