MTHFR antibody (AA 1-325)
Quick Overview for MTHFR antibody (AA 1-325) (ABIN7436504)
Target
See all MTHFR AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 1-325
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Purpose
- Polyclonal Antibody to Methylenetetrahydrofolate Reductase (MTHFR)
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Specificity
- The antibody is a rabbit polyclonal antibody raised against MTHFR. It has been selected for its ability to recognize MTHFR in immunohistochemical staining and western blotting.
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Cross-Reactivity
- Mouse, Rat
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Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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Immunogen
- Recombinant Methylenetetrahydrofolate Reductase (MTHFR) corresdonding to Met1~Arg325
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Isotype
- IgG
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Application Notes
- Western blotting: 0.5-2 μg/mL,1:380-1500,Immunohistochemistry: 5-20 μg/mL,1:38-150,Immunocytochemistry: 5-20 μg/mL,1:38-150,Optimal working dilutions must be determined by end user.
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Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.76 mg/mL
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Buffer
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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Expiry Date
- 24 months
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- MTHFR (Methylenetetrahydrofolate Reductase (NAD(P)H) (MTHFR))
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Alternative Name
- MTHFR
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Background
- 5,10-Methylenetetrahydrofolate Reductase(NADPH)
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UniProt
- P42898
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Pathways
- Methionine Biosynthetic Process
Target
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