Klotho antibody (AA 517-956)

Catalog No. ABIN7436552

This Rabbit Polyclonal antibody specifically detects Klotho in WB, IHC, IP and ICC. It exhibits reactivity toward Rat.

Quick Overview for Klotho antibody (AA 517-956) (ABIN7436552)

Target: Klotho (KL)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Klotho antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Klotho Antibody

Binding Specificity: AA 517-956

Purpose: Polyclonal Antibody to Klotho (KL)

Specificity: The antibody is a rabbit polyclonal antibody raised against KL. It has been selected for its ability to recognize KL in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Klotho (KL) corresdonding to Leu517~Leu956 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.36 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Klotho

Target: Klotho (KL)

Alternative Name: Klotho

Background: KLa, Klotho Alpha

UniProt: Q9Z2Y9

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Activity, Growth Factor Binding