IDH2 antibody (AA 40-452)

Catalog No. ABIN7441520

The Rabbit Polyclonal anti-IDH2 antibody has been validated for WB and IHC. It is suitable to detect IDH2 in samples from Human.

Quick Overview for IDH2 antibody (AA 40-452) (ABIN7441520)

Target: IDH2 (Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IDH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-IDH2 Antibody

Binding Specificity: AA 40-452

Purpose: Polyclonal Antibody to Isocitrate Dehydrogenase 2, mitochondrial (IDH2)

Specificity: The antibody is a rabbit polyclonal antibody raised against IDH2. It has been selected for its ability to recognize IDH2 in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Isocitrate Dehydrogenase 2, mitochondrial (IDH2) corresdonding to Ala40~Gln452 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for IDH2

Target: IDH2 (Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2))

Alternative Name: IDH2

Background: IDH, IDP, ICD-M, IDHM, mNADP-IDH, Isocitrate dehydrogenase [NADP], mitochondrial, Oxalosuccinate decarboxylase

UniProt: P48735

Pathways: Warburg Effect