EEA1 antibody (AA 1232-1348)

Catalog No. ABIN7441538

This Rabbit Polyclonal antibody specifically detects EEA1 in WB, IHC, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for EEA1 antibody (AA 1232-1348) (ABIN7441538)

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-EEA1 Antibody

Binding Specificity: AA 1232-1348

Purpose: Polyclonal Antibody to Early Endosome Antigen 1 (EEA1)

Specificity: The antibody is a rabbit polyclonal antibody raised against EEA1. It has been selected for its ability to recognize EEA1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Early Endosome Antigen 1 (EEA1) corresdonding to His1232~Lys1348 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blottin:g: 0.01-2 μg/mL,Immunohistochemistry: 2-20 μg/mL,Immunocytochemistry: 2-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for EEA1

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Alternative Name: EEA1

Background: ZFYVE2, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2

UniProt: Q15075

Pathways: Toll-Like Receptors Cascades