BCKDK antibody (AA 44-223)

Catalog No. ABIN7436711

This Rabbit Polyclonal antibody specifically detects BCKDK in WB, IHC, ICC and IP. It exhibits reactivity toward Rat.

Quick Overview for BCKDK antibody (AA 44-223) (ABIN7436711)

Target: BCKDK (Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCKDK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-BCKDK Antibody

Binding Specificity: AA 44-223

Purpose: Polyclonal Antibody to Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK)

Specificity: The antibody is a rabbit polyclonal antibody raised against BCKDK. It has been selected for its ability to recognize BCKDK in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) corresdonding to Arg44~Thr223 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for BCKDK

Target: BCKDK (Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK))

Alternative Name: BCKDK

Background: BCKDHKIN, 3-Methyl-2-Oxobutanoate Dehydrogenase/Lipoamide Kinase,Mitochondrial

UniProt: Q00972

Pathways: SARS-CoV-2 Protein Interactome