GLI3 antibody (AA 303-561)

Catalog No. ABIN7439492

This Rabbit Polyclonal antibody specifically detects GLI3 in WB, IHC, ICC and IP. It exhibits reactivity toward Mouse.

Quick Overview for GLI3 antibody (AA 303-561) (ABIN7439492)

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-GLI3 Antibody

Binding Specificity: AA 303-561

Purpose: Polyclonal Antibody to GLI Family Zinc Finger Protein 3 (GLI3)

Specificity: The antibody is a rabbit polyclonal antibody raised against GLI3. It has been selected for its ability to recognize GLI3 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant GLI Family Zinc Finger Protein 3 (GLI3) corresdonding to Ser303~Glu561 (Accession # Q61602)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for GLI3

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Alternative Name: GLI3

Background: GCPS, PHS, PAP-A, PAPA, PAPA1, PAPB, ACLS, PPDIV, Greig Cephalopolysyndactyly Syndrome, Glioma-Associated Oncogene Family Zinc Finger 3, GLI3 form of 190 kDa

UniProt: Q61602

Pathways: Hedgehog Signaling