PIK3CD antibody (AA 774-1044)

Catalog No. ABIN7436747

The Rabbit Polyclonal anti-PIK3CD antibody is suitable to detect PIK3CD in samples from Human. It has been validated for WB, ELISA, IHC (p), ICC and IHC (fro).

Quick Overview for PIK3CD antibody (AA 774-1044) (ABIN7436747)

Target: PIK3CD (Phosphoinositide-3-Kinase, Catalytic, delta Polypeptide (PIK3CD))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3CD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PIK3CD Antibody

Binding Specificity: AA 774-1044

Purpose: Polyclonal Antibody to Phosphoinositide-3-Kinase Catalytic Delta Polypeptide (PIK3Cd)

Specificity: The antibody is a rabbit polyclonal antibody raised against PIK3Cd. It has been selected for its ability to recognize PIK3Cd in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Phosphoinositide-3-Kinase Catalytic Delta Polypeptide (PIK3Cd) corresdonding to Val774~Gln1044 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 1-5 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PIK3CD

Target: PIK3CD (Phosphoinositide-3-Kinase, Catalytic, delta Polypeptide (PIK3CD))

Alternative Name: PIK3Cd

Background: P110D, P110δ, Phosphoinositide-3-Kinase C, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta

UniProt: O00329

Pathways: BCR Signaling, Warburg Effect