NOTCH3 antibody (AA 2053-2318)

Catalog No. ABIN7442738

This Rabbit Polyclonal antibody specifically detects NOTCH3 in WB, IHC, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for NOTCH3 antibody (AA 2053-2318) (ABIN7442738)

Target: NOTCH3 (Notch 3 (NOTCH3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOTCH3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-NOTCH3 Antibody

Binding Specificity: AA 2053-2318

Purpose: Polyclonal Antibody to Notch Homolog 3 (NOTCH3)

Specificity: The antibody is a rabbit polyclonal antibody raised against NOTCH3. It has been selected for its ability to recognize NOTCH3 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Notch Homolog 3 (NOTCH3) corresdonding to Leu2053~Gln2318 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:250-1000,Immunohistochemistry: 5-20 μg/mL,1:25-100,Immunocytochemistry: 5-20 μg/mL,1:25-100,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for NOTCH3

Target: NOTCH3 (Notch 3 (NOTCH3))

Alternative Name: NOTCH3

Background: CADASIL, CASIL, Neurogenic locus notch homolog protein 3

UniProt: Q9UM47

Pathways: Notch Signaling