NRF2 antibody (AA 400-603)

Catalog No. ABIN7441673

The Rabbit Polyclonal anti-NRF2 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect NRF2 in samples from Human.

Quick Overview for NRF2 antibody (AA 400-603) (ABIN7441673)

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NRF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-NRF2 Antibody

Binding Specificity: AA 400-603

Purpose: Polyclonal Antibody to Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2)

Specificity: The antibody is a rabbit polyclonal antibody raised against NFE2L2. It has been selected for its ability to recognize NFE2L2 in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) corresdonding to Ser400~Lys603 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for NRF2

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Alternative Name: NFE2L2

Background: NRF2, HEBP1, NF-E2-Related Factor 2, Nuclear factor, erythroid derived 2, like 2

UniProt: Q16236

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling