HIF1AN antibody

Catalog No. ABIN7239416

The Rabbit Polyclonal anti-HIF1AN antibody is suitable to detect HIF1AN in samples from Human and Mouse. It has been validated for WB, ELISA and IHC.

Quick Overview for HIF1AN antibody (ABIN7239416)

Target: HIF1AN (Hypoxia Inducible Factor 1, alpha Subunit Inhibitor (HIF1AN))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIF1AN antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-HIF1AN Antibody

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: Synthetic peptide of human HIF1AN

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:300

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.6 mg/mL

Buffer: PBS with 0.05 % sodium azide and 50 % glycerol, PH7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for HIF1AN

Target: HIF1AN (Hypoxia Inducible Factor 1, alpha Subunit Inhibitor (HIF1AN))

Alternative Name: HIF1AN

Background: Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1alpha. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the beta-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1alpha. This hydroxylation event blocks the association of HIF-1alpha with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1alpha. In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm.

Molecular Weight: 40 kDa

NCBI Accession: NP_060372

UniProt: Q9NWT6

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development