Sonic Hedgehog antibody

Catalog No. ABIN7242410

The Rabbit Polyclonal anti-Sonic Hedgehog antibody (ABIN7242410) specifically detects Sonic Hedgehog in IHC and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Sonic Hedgehog antibody (ABIN7242410)

Target: Sonic Hedgehog (SHH)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sonic Hedgehog antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Product Details anti-Sonic Hedgehog Antibody

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: Synthetic peptide of human SHH

Isotype: IgG

Application Details

Application Notes: IHC 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.8 mg/mL

Buffer: PBS with 0.05 % sodium azide and 50 % glycerol, PH7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Alternative Name: SHH

Background: This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities.

NCBI Accession: NP_000184

UniProt: Q15465

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development