ECH1 antibody

Catalog No. ABIN7247000

This Rabbit Polyclonal antibody specifically detects ECH1 in WB, ELISA and IHC. It exhibits reactivity toward Human and Rat.

Quick Overview for ECH1 antibody (ABIN7247000)

Target: ECH1 (Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ECH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-ECH1 Antibody

Characteristics: Polyclonal Antibody

Purification: Antigen affinity purification

Immunogen: Fusion protein of human ECH1

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:300, ELISA 1:5000-1:10000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.6 mg/mL

Buffer: PBS with 0.05 % Sodium azide and 40 % Glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for ECH1

Target: ECH1 (Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1))

Alternative Name: ECH1

Background: This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

Molecular Weight:

Observed_MW: Refer to figures

Calculated_MW: 36 kDa

UniProt: Q13011

Pathways: Monocarboxylic Acid Catabolic Process