Cullin 4A antibody

Catalog No. ABIN7247885

This anti-Cullin 4A antibody is a Rabbit Polyclonal antibody detecting Cullin 4A in WB, ELISA and IHC. Suitable for Human and Mouse.

Quick Overview for Cullin 4A antibody (ABIN7247885)

Target: Cullin 4A (CUL4A)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 4A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-Cullin 4A Antibody

Characteristics: Polyclonal Antibody

Purification: Antigen affinity purification

Immunogen: Synthetic peptide of human CUL4A

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:300, ELISA 1:5000-1:10000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.62 mg/mL

Buffer: PBS with 0.05 % Sodium azide and 40 % Glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Cullin 4A

Target: Cullin 4A (CUL4A)

Alternative Name: CUL4A

Background: Cullin proteins assemble a large number of RING E3 ubiquitin ligases,participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins,CUL4A (87 kDa) and CUL4B(104 kDa),have been identified. The two CUL4 sequences are 83 % identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases,DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol,suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2.

Molecular Weight:

Observed_MW: Refer to figures

Calculated_MW: 88 kDa

UniProt: Q13619