GLI1 antibody

Catalog No. ABIN7248113

The Rabbit Polyclonal anti-GLI1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect GLI1 in samples from Human and Mouse.

Quick Overview for GLI1 antibody (ABIN7248113)

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-GLI1 Antibody

Characteristics: Polyclonal Antibody

Purification: Antigen affinity purification

Immunogen: Synthetic peptide of human GLI1

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:100, ELISA 1:5000-1:10000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.08 mg/mL

Buffer: PBS with 0.05 % Sodium azide and 40 % Glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for GLI1

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Alternative Name: GLI1

Background: GLI1 (GLI Family Zinc Finger 1) is a Protein Coding gene. Diseases associated with GLI1 include Basal Cell Carcinoma and Necrobiosis Lipoidica. Among its related pathways are Wnt Signaling Pathways: beta-Catenin-independent Wnt/Ca2+ Signaling and Other Non-canonical Wnt Signaling Pathways and G-Beta Gamma Signaling. GO annotations related to this gene include chromatin binding and microtubule binding. An important paralog of this gene is GLI2. This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight:

Observed_MW: Refer to figures

Calculated_MW: 118 kDa

UniProt: P08151

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis