Cytochrome C antibody

Catalog No. ABIN7249416

This Rabbit Polyclonal antibody specifically detects Cytochrome C in WB, IHC and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Cytochrome C antibody (ABIN7249416)

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytochrome C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Cytochrome C Antibody

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse Cytochrome c

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:500-1:1000, IF 1:200-1:800

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.5 mg/mL

Buffer: PBS with 0.02 % sodium azide, 1 % BSA and 50 % glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Alternative Name: CYCS

Background: Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation,cytochrome c can be released from mitochondria into cytoplasm,resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition,cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy.

Molecular Weight:

Observed_MW: 12 kDa

Calculated_MW: 12 kDa

UniProt: P99999, P62898, P62897

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity