Dihydrofolate Reductase antibody

Catalog No. ABIN7253152

The Rabbit Polyclonal anti-Dihydrofolate Reductase antibody has been validated for WB, ELISA and IHC. It is suitable to detect Dihydrofolate Reductase in samples from Human, Mouse and Rat.

Quick Overview for Dihydrofolate Reductase antibody (ABIN7253152)

Target: Dihydrofolate Reductase (DHFR)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dihydrofolate Reductase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-Dihydrofolate Reductase Antibody

Characteristics: Polyclonal Antibody

Purification: Antigen affinity purification

Immunogen: Fusion protein of human DHFR

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:300, ELISA 1:5000-1:10000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.96 mg/mL

Buffer: PBS with 0.05 % Sodium azide and 40 % Glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Dihydrofolate Reductase

Target: Dihydrofolate Reductase (DHFR)

Alternative Name: DHFR

Background: Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene.

Molecular Weight:

Observed_MW: Refer to figures

Calculated_MW: 21 kDa

UniProt: P00374

Pathways: Mitotic G1-G1/S Phases