RIPK3 antibody (Cy5)

Catalog No. ABIN700679

The Rabbit Polyclonal anti-RIPK3 antibody has been validated for WB and IF (p). It is suitable to detect RIPK3 in samples from Human, Mouse and Rat.

Quick Overview for RIPK3 antibody (Cy5) (ABIN700679)

Target: RIPK3 (Receptor-Interacting serine-threonine Kinase 3 (RIPK3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIPK3 antibody is conjugated to Cy5

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-RIPK3 Antibody (Cy5)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RIPK3

Isotype: IgG

Application Details

Application Notes: IF(IHC-P): (1:50-200)
Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RIPK3

Target: RIPK3 (Receptor-Interacting serine-threonine Kinase 3 (RIPK3))

Alternative Name: Ripk3

Background:

Synonyms: Receptor interacting protein 3, Receptor interacting serine threonine kinase 3, Receptor interacting serine/threonine protein kinase 3, Receptor-interacting protein 3, Receptor-interacting serine/threonine-protein kinase 3, RIP 3, RIP like protein kinase 3, RIP-3, RIP-like protein kinase 3, RIPK 3, RIPK3, RIPK3_HUMAN.

Background: Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains an N terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-kB activation. Over expression of RIP3 induces apoptosis and NF-kB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues.

Gene ID: 11035

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades