Cofilin antibody (pSer3)

Catalog No. ABIN7265124

The Rabbit Polyclonal anti-Cofilin antibody (ABIN7265124) specifically detects Cofilin in WB, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Cofilin antibody (pSer3) (ABIN7265124)

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cofilin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Cofilin Antibody

Binding Specificity: pSer3

Characteristics: Phosphorylated antibody

Purification: Affinity purification

Immunogen: A phospho specific peptide corresponding to residues surrounding S3 of human CFL1

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:100 IF 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Alternative Name: CFL1

Background: The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH -dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH -dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.

Molecular Weight:

Observed_MW: 18 kDa

Calculated_MW: 18 kDa

Gene ID: 1072

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events