POLR2A/RPB1 antibody (pSer5)

Catalog No. ABIN7265278

This Rabbit Polyclonal antibody specifically detects POLR2A/RPB1 in WB, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for POLR2A/RPB1 antibody (pSer5) (ABIN7265278)

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2A/RPB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-POLR2A/RPB1 Antibody

Binding Specificity: pSer5

Characteristics: Phosphorylated antibody

Purification: Affinity purification

Immunogen: A phospho specific peptide corresponding to residues surrounding S5 of human POLR2A

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:100 IF 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Alternative Name: POLR2A

Background: This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Molecular Weight:

Observed_MW: 260 kDa

Calculated_MW: 63 kDa/217 kDa

Gene ID: 5430

UniProt: P24928

Pathways: Regulatory RNA Pathways