CYBB antibody (Internal Region)

Catalog No. ABIN7013861

The Goat Polyclonal anti-CYBB antibody (ABIN7013861) specifically detects CYBB in WB and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for CYBB antibody (Internal Region) (ABIN7013861)

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CYBB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CYBB Antibody

Binding Specificity: Internal Region

Purpose: GP91-PHOX / NOX2

Sequence: CDKQEFQDAW KLPK

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Application Details

Application Notes:

Western Blot: Approx 75 kDa band observed in lysates of cell line Daudi and in Mouse Spleen lysates (calculated MW of 65.3 kDa according to Human NP_000388.2 and Mouse NP_031833.3). This band was successfully blocked by incubation with the mmunising peptid

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CYBB

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Alternative Name: CYBB

Background: CYBB, cytochrome b-245, beta polypeptide, CGD, GP91-1, GP91-PHOX, GP91PHOX, NOX2, p91-PHOX, CGD91-phox, NADPH oxidase 2, cytochrome b(558) subunit beta, cytochrome b-245 heavy chain, cytochrome b558 subunit beta, heme-binding membrane glycoprotein gp91pho

Gene ID: 1536, 13058, 66021