SCRIB antibody (Internal Region)

Catalog No. ABIN7013862

This Goat Polyclonal antibody specifically detects SCRIB in WB, ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for SCRIB antibody (Internal Region) (ABIN7013862)

Target: SCRIB (Scribbled Homolog (SCRIB))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SCRIB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-SCRIB Antibody

Binding Specificity: Internal Region

Purpose: SCRIB / Scribble

Sequence: PEGPGKEKEL PGQ

Specificity: This antibody is expected to recognize both reported isoforms (NP_874365.3, NP_056171.3).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Application Details

Application Notes:

Western Blot: Approx 200-250 kDa band observed in lysates of cell lines HeLa, HepG2 and U2OS (calculated MW of 178 kDa according to NP_874365.3). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3 μg/mL. Primary inc

Peptide ELISA: antibody detection limit dilution 1:1000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cell junctions of MCF7 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SCRIB

Target: SCRIB (Scribbled Homolog (SCRIB))

Alternative Name: SCRIB

Background: SCRIB, scribbled homolog (Drosophila), CRIB1, SCRB1, SCRIB1, Vartul, scribble

Gene ID: 23513

Pathways: Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Tube Formation, Synaptic Vesicle Exocytosis, Asymmetric Protein Localization