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SCRIB antibody (Internal Region)

Verified SCRIB Reactivity: Human WB, ELISA, IF Host: Goat Polyclonal unconjugated
Catalog No. ABIN7013862
  • Target See all SCRIB Antibodies
    SCRIB (Scribbled Homolog (SCRIB))
    Binding Specificity
    • 7
    • 7
    • 6
    • 3
    • 1
    • 1
    Internal Region
    Reactivity
    • 28
    • 8
    • 8
    • 1
    Human
    Host
    • 27
    • 1
    Goat
    Clonality
    • 28
    Polyclonal
    Conjugate
    • 10
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SCRIB antibody is un-conjugated
    Application
    • 18
    • 15
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF)
    Purpose
    SCRIB / Scribble
    Sequence
    PEGPGKEKEL PGQ
    Specificity
    This antibody is expected to recognize both reported isoforms (NP_874365.3, NP_056171.3).
    Cross-Reactivity
    Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Top Product
    Discover our top product SCRIB Primary Antibody
  • Application Notes

    Western Blot: Approx 200-250 kDa band observed in lysates of cell lines HeLa, HepG2 and U2OS (calculated MW of 178 kDa according to NP_874365.3). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3 μg/mL. Primary inc

    Peptide ELISA: antibody detection limit dilution 1:1000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the cell junctions of MCF7 cells. Recommended concentration: 10μg/ml.

    Restrictions
    For Research Use only
  • Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    SCRIB (Scribbled Homolog (SCRIB))
    Alternative Name
    SCRIB (SCRIB Products)
    Synonyms
    0424/05 antibody, CG31082 antibody, CG42614 antibody, CG43398 antibody, CG5462 antibody, CG5467 antibody, CT17324 antibody, Dmel\\CG43398 antibody, Dmel_CG31082 antibody, Dmel_CG42614 antibody, Dmel_CG5462 antibody, Dmel_CG5467 antibody, MENE (3R)-F antibody, SCR antibody, Scb antibody, Scrb antibody, Scrib antibody, ird antibody, ird15 antibody, l(3)673 antibody, l(3)S042405 antibody, l(3)c00119 antibody, l(3)j7B3 antibody, scrb antibody, smi97B antibody, vart antibody, AI118201 antibody, CRIB antibody, Crc antibody, SCRIB1 antibody, Scrb1 antibody, mKIAA0147 antibody, vartul antibody, scrb1 antibody, scribble antibody, scribble1 antibody, si:ch211-12p8.1 antibody, si:zc12p8.1 antibody, CRIB1 antibody, SCRB1 antibody, Vartul antibody, scribbled antibody, scribbled planar cell polarity antibody, scribbled planar cell polarity protein antibody, scrib antibody, Scrib antibody, SCRIB antibody
    Background
    SCRIB, scribbled homolog (Drosophila), CRIB1, SCRB1, SCRIB1, Vartul, scribble
    Gene ID
    23513
    Pathways
    Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Tube Formation, Synaptic Vesicle Exocytosis, Asymmetric Protein Localization
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