GATA1 antibody (C-Term)

Catalog No. ABIN7013869

The Goat Polyclonal anti-GATA1 antibody has been validated for WB, ELISA, IF and FACS. It is suitable to detect GATA1 in samples from Human.

Quick Overview for GATA1 antibody (C-Term) (ABIN7013869)

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GATA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-GATA1 Antibody

Binding Specificity: C-Term

Purpose: GATA1

Sequence: PTTSTTVVAP LSS

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Application Details

Application Notes:

Western Blot: Approx 50 kDa band observed in nuclear lysates of cell line K562 (calculated MW of 42.8 kDa according to NP_002040.1). The observed molecular weight corresponds to different antibodies from other sources and was successfully blocked by incuba

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GATA1

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Alternative Name: GATA1

Background: GATA1, GATA binding protein 1 (globin transcription factor 1), GF1, NFE1, ERYF1

Gene ID: 2623

Pathways: Cellular Response to Molecule of Bacterial Origin