CTNNB1 antibody (Internal Region)

Catalog No. ABIN7013874

This Goat Polyclonal antibody specifically detects CTNNB1 in WB, ELISA, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for CTNNB1 antibody (Internal Region) (ABIN7013874)

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CTNNB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-CTNNB1 Antibody

Binding Specificity: Internal Region

Purpose: CTNNB1 / catenin beta-1

Sequence: ETARCTAGTL HNLS

Specificity: Reported variants represent identical protein: NP_001091679.1, NP_001895.1, NP_001091680.1.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Application Details

Application Notes:

Western Blot: Approx 90 kDa band observed in A431 cell lysates-and in preliminary testing of CACO2 cell lysate (calculated MW of 85.5 kDa according to NP_001895.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of U251 and A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended c

Restrictions: For Research Use only

Handling

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CTNNB1

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Alternative Name: CTNNB1

Background: CTNNB1, catenin (cadherin-associated protein), beta 1, 88 kDa, CTNNB, MRD19, armadillo, catenin beta-1

Gene ID: 1499, 12387, 84353

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2