VEGFA antibody (AA 387-399)

Catalog No. ABIN7013875

The Goat Polyclonal anti-VEGFA antibody has been validated for WB and ELISA. It is suitable to detect VEGFA in samples from Human and Mouse.

Quick Overview for VEGFA antibody (AA 387-399) (ABIN7013875)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This VEGFA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-VEGFA Antibody

Binding Specificity: AA 387-399

Purpose: VEGFA (aa387-399)

Sequence: KNTDSRCKAR QLE

Specificity: This antibody is expected to recognize following isoforms: a (45.5 kDa), b (43.6 kDa), c (42.8 kDa), d (40.7 kDa), g (40.6 kDa), i (27.0 kDa), j (25.2 kDa), k (24.4 kDa), o (22.3 kDa) and s (18.9 kDa)

Cross-Reactivity: Cat, Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Application Details

Application Notes:

Western Blot: Approx 38 kDa band observed in Mouse Liver lysates,38+28+18 kDa in Human Kidney lysates and approx 18 kDa in lysates of cell line K562 (calculated MW of 40.3 kDa according to Mouse NP_033531.3, 40.6 kDa according to Human NP_001028928.1, 27.0 kDa

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: VEGFA

Background: VEGFA, vascular endothelial growth factor A, MGC70609, MVCD1, VEGF, VPF, OTTHUMP00000016487, OTTHUMP00000016488, OTTHUMP00000165985, OTTHUMP00000165986, OTTHUMP00000165987, OTTHUMP00000224107, OTTHUMP00000224108, OTTHUMP00000224109, OTTHUMP00000224153, OT

Gene ID: 7422, 22339, 83785

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling