CD8B antibody

Catalog No. ABIN7013937

This Mouse Monoclonal antibody specifically detects CD8B in FACS, WB, IP, IHC (fro) and Func. It exhibits reactivity toward Rat.

Quick Overview for CD8B antibody (ABIN7013937)

Target: CD8B (CD8b Molecule (CD8B))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8B antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: 341

Product Details anti-CD8B Antibody

Purpose: Anti-Rt CD8b Purified Low Endotoxin

Specificity: The mouse monoclonal antibody 341 (also known as 34.1) recognizes rat CD8b, the 32-34 kDa beta chain of the CD8 coreceptor (extracellular epitope), expressed on T cell subsets and some other cell types, such as macrophages.

Purification: Purified by protein-A affinity chromatography.

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: CD8 positive Wistar rat splenic T cell hybridomas

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.
Functional application: In vitro stimulation, in vivo depletion.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for CD8B

Target: CD8B (CD8b Molecule (CD8B))

Alternative Name: CD8b

Background: CD8b molecule,The CD8b (CD8 beta) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.,Ly-3, Ly-C, Lyt-3

Gene ID: 926

UniProt: P10966

Pathways: TCR Signaling