ITGA5 antibody

Catalog No. ABIN7013950

The Rat Monoclonal anti-ITGA5 antibody is suitable to detect ITGA5 in samples from Mouse. It has been validated for FACS, ICC and IHC (fro).

Quick Overview for ITGA5 antibody (ABIN7013950)

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGA5 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 5H10-27 (MFR5)

Product Details anti-ITGA5 Antibody

Purpose: Anti-Ms CD49e Purified

Characteristics: The rat monoclonal antibody 5H10-27 (MFR5) recognizes an extracellular epitope of murine CD49e, a 135 kDa protein serving as VLA-5 alpha chain, expressed on thymocytes, activated T cells, splenic B cells, and mast cells.

Purification: Purified by protein-G affinity chromatography.

Immunogen: C57BL/6 x A/J)F1 murine mast cell line

Isotype: IgG2a, kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for ITGA5

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Alternative Name: CD49e

Background: Integrin alpha 5,CD49e, the alpha 5 integrin, noncovalently associates with the beta 1integrin (CD29) to form VLA-5 integrin complex. CD49e itself is composed of two disulfide-linked chains of 135 kDa and 25 kDa. VLA-5 binds to RGD sequence of fibronectin, and to neural adhesion molecule L1. It is important in maintaining the integrity of endothelial monolayers, in monocyte migration into the extracellular tissues, and it also provides a co-stimulatory signal to T cells and enhances receptor and complement receptor-mediated phagocytosis.,ITGA5, VLA-5 alpha, FNRA

Gene ID: 16402

UniProt: P11688

Pathways: Integrin Complex