ULK1 antibody (AA 951-1050)

Catalog No. ABIN701440

This Rabbit Polyclonal antibody specifically detects ULK1 in WB, FACS, IF (cc), IF (p), IHC (p) and IHC (fro). It exhibits reactivity toward Human. It has been mentioned in 3+ publications

Quick Overview for ULK1 antibody (AA 951-1050) (ABIN701440)

Target: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ULK1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ULK1 Antibody

Binding Specificity: AA 951-1050

Purpose: ATG1/ULK1 Polyclonal Antibody

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ATG1/ULK1

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000),

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-ULK1 antibody (ABIN701440)

Shen, Hou, Zhu, Zhao, Ouyang, Feng et al.: "Cortical spreading depression preconditioning mediates neuroprotection against ischemic stroke by inducing AMP-activated protein kinase-dependent autophagy in a rat cerebral ischemic/reperfusion ..." in: Journal of neurochemistry, Vol. 140, Issue 5, pp. 799-813, (2016) (PubMed).

Gao, Wang, Yi, Kou, Xie, Song: "Activation of lysosomal degradative pathway in spinal cord tissues of carbon disulfide-treated rats." in: Chemico-biological interactions, Vol. 219C, pp. 76-82, (2014) (PubMed).

Song, Kou, Zou, Gao, Zeng, Xie: "Involvement of autophagy in tri-ortho-cresyl phosphate- induced delayed neuropathy in hens." in: Neurochemistry international, Vol. 64, pp. 1-8, (2013) (PubMed).

Target Details for ULK1

Target: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Alternative Name: ATG1/ULK1

Background:

Synonyms: ATG1, ATG1A, UNC51, hATG1, Unc51.1, Serine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, Unc-51-like kinase 1, ULK1, KIAA722

Background: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA652 and RPTOR, however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation.

Gene ID: 8408

UniProt: O75385

Pathways: Regulation of Cell Size, Autophagy