FLASH antibody (AA 1861-1982)

Catalog No. ABIN702220

This Rabbit Polyclonal antibody specifically detects FLASH in WB, ELISA, IF (cc), IF (p), IHC (fro) and IHC (p). It exhibits reactivity toward Mouse.

Quick Overview for FLASH antibody (AA 1861-1982) (ABIN702220)

Target: FLASH (CASP8AP2) (CASP8 Associated Protein 2 (CASP8AP2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLASH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FLASH Antibody

Binding Specificity: AA 1861-1982

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FLASH/CASP8AP2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for FLASH

Target: FLASH (CASP8AP2) (CASP8 Associated Protein 2 (CASP8AP2))

Alternative Name: FLASH/CASP8AP2

Background:

Synonyms: CASP 8 associated protein 2, CASP8 associated protein 2, CASP8AP2, Caspase 8 associated protein 2, CED 4, CED4, FLASH homolog, FLASH homolog RIP 25, FLASH homolog RIP25, FLICE associated huge, FLICE associated huge protein, FLICE-associated huge protein, RIP 25, RIP25, AA387232, D4Ertd659e, mKIAA1315, C8AP2_HUMAN, CASP8-associated protein 2, FLICE-associated huge protein, FLJ11208, KIAA1315, OTTMUSP00000005125, RP23-69G24.1.

Background: FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8. There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity.Studies of FLASH protein suggested that this protein may be a component of the death inducing signaling complex that includes Fas receptor, Fas binding adapter FADD, and caspase 8, and plays a regulatory role in Fas mediated apoptosis.

Gene ID: 9994

Pathways: Chromatin Binding, Positive Regulation of Endopeptidase Activity