POU4F1 antibody (AA 325-419)

Catalog No. ABIN702400

This Rabbit Polyclonal antibody specifically detects POU4F1 in WB, ELISA, FACS, IF (cc), IF (p), IHC (fro) and IHC (p). It exhibits reactivity toward Human, Mouse, Rat and Pig. It has been mentioned in 2+ publications

Quick Overview for POU4F1 antibody (AA 325-419) (ABIN702400)

Target: POU4F1 (POU Domain, Class 4, Transcription Factor 1 (POU4F1))

Reactivity: Human, Mouse, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POU4F1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-POU4F1 Antibody

Binding Specificity: AA 325-419

Cross-Reactivity: Human, Mouse, Pig, Rat

Predicted Reactivity: Dog,Cow,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human BRN3A

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-POU4F1 antibody (ABIN702400)

Xue, Qian, Hu, Zhou, Zhou, Hu, Karakhanyan, Pang, Fu: "Sequential regulatory loops as key gatekeepers for neuronal reprogramming in human cells." in: Nature neuroscience, Vol. 19, Issue 6, pp. 807-15, (2016) (PubMed).

Bell, Wilding, Funke, Perumal, Beck, Wolters, Holz-Müller, Pfeiffer, Grus: "Neuroprotective effects of antibodies on retinal ganglion cells in an adolescent retina organ culture." in: Journal of neurochemistry, Vol. 139, Issue 2, pp. 256-269, (2016) (PubMed).

Target Details for POU4F1

Target: POU4F1 (POU Domain, Class 4, Transcription Factor 1 (POU4F1))

Alternative Name: BRN3A

Background:

Synonyms: BRN3A, RDC-1, Oct-T1, brn-3A, POU domain, class 4, transcription factor 1, Brain-specific homeobox/POU domain protein 3A, Brain-3A, Homeobox/POU domain protein RDC-1, POU4F1, RDC1

Background: Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons.

Gene ID: 5457

UniProt: Q01851

Pathways: Feeding Behaviour