ADAM22 antibody (Extracellular, N-Term)

Catalog No. ABIN7886666

The Rabbit Polyclonal anti-ADAM22 antibody (ABIN7886666) specifically detects ADAM22 in WB, IHC and IF. The antibody is reactive with Mouse samples.

Quick Overview for ADAM22 antibody (Extracellular, N-Term) (ABIN7886666)

Target: ADAM22 (ADAM Metallopeptidase Domain 22 (ADAM22))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADAM22 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ADAM22 Antibody

Binding Specificity: AA 715-729, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to ADAM22

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-ADAM22 (extracellular) Antibody (ABIN7042881, ABIN7044724 and ABIN7044725) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize ADAM22 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)HNDDAKTGITLSGNG, corresponding to amino acid residues 715-729 of mouse ADAM22

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7234619)

Blocking Peptide: (ABIN7234619)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for ADAM22

Target: ADAM22 (ADAM Metallopeptidase Domain 22 (ADAM22))

Alternative Name: ADAM22

Background:

Synonyms: Disintegrin and metalloproteinase domain-containing protein 22, ADAM metallopeptidase domain 22, MDC2

Description: ADAMs (a disintegrin and metalloproteinases) are multi-domain transmembrane glycoproteins with diverse roles in physiology and disease. Notably, 8 of 21 ADAMs lack functional metalloproteinase domains and are implicated in protein-protein interactions instead of membrane protein ectodomain shedding.ADAM22 is a non-proteinase which acts as a post synaptic receptor for the secreted neurotransmission modulator LGI-1 at neural synapses. ADAM22 is a compact four-leaf clover with the metalloproteinase-like domain (Domain M) held in the concave face of a rigid module formed by the disintegrin (Domain D), cysteine-rich (Domain C), and epidermal growth factor-like domains (Domain E). The largest domain in the four-leaf clover, domain M, is distal to the cell membrane. Following domain M, domain D and domain C zigzag to domain E in a compact, but not extended fashion. A 15-amino acid linker, leads the C terminus of domain E to the membrane. The loss of metalloproteinase activity is ensured by the absence of critical catalytic residues, the filling of the substrate groove, and the steric hindrance by the cysteine-rich domain. The extracellular domain of ADAM22 interacts with LGI-1, whereas its cytoplasmic PDZ-binding motif recruits PSD-95. The link of ADAM22 and LGI-1 to AMPA receptors establishes their roles in glutamate neurotransmission1.Mutations that impair LGI-1 binding to ADAM22 are implicated in the pathogenesis of Autosomal dominant lateral temporal epilepsy (ADTLE), a focal epilepsy syndrome characterized by focal seizures with prominent auditory or aphasic symptoms, normal magnetic resonance imaging, and usually benign evolution2.

Gene ID: 11496

NCBI Accession: NM_021723

UniProt: Q9R1V6