Adenosine A3 Receptor antibody (3rd Intracellular Loop)

Catalog No. ABIN7042891

This Rabbit Polyclonal antibody specifically detects Adenosine A3 Receptor in WB, IF, IHC and IC. It exhibits reactivity toward Human.

Quick Overview for Adenosine A3 Receptor antibody (3rd Intracellular Loop) (ABIN7042891)

Target: Adenosine A3 Receptor (ADORA3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Adenosine A3 Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunochromatography (IC)

Product Details anti-Adenosine A3 Receptor Antibody

Binding Specificity: 3rd Intracellular Loop, AA 216-230

Purpose: A Rabbit Polyclonal Antibody to Adenosine A3 Receptor

Cross-Reactivity: Human, Rat

Predicted Reactivity: Rat,mouse - 14,16 amino acid residues identical

Characteristics: Anti-Adenosine A3 Receptor Antibody is directed against an intracellular epitope of the human adenosine A3 receptor (A3AR). Anti-Adenosine A3 Receptor Antibody (ABIN7042891, ABIN7043892 and ABIN7043893) can be used in western blot analysis, as well as immunocytochemical and immunohistocemical applications, and has been designed to recognize A3AR from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KETGAFYGREFKTAK, corresponding to amino acid residues 216-230 of human A3AR

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: 1:100

IHC: 1:50

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7234639)

Blocking Peptide: (ABIN7234639)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Adenosine A3 Receptor

Target: Adenosine A3 Receptor (ADORA3)

Alternative Name: ADORA3

Background:

Synonyms: ADORA3, A3AR, Adenosine A3R

Description: Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart.1,2 Adenosine exerts its action via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). The various adenosine receptors can be distinguished on the basis of their distinct molecular structures, distinct tissue distributions, and differential selectivity for adenosine analogs.1-4 All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop important in binding G proteins.1-3Expression of A3AR has been reported in the brain, kidney, liver, and heart. It plays a role in modulation of cerebral ischemia, asthma, and cell growth. In addition, recent studies have established a cardioprotective role for A3AR.5 Expression of A3AR was reported to be elevated in cancerous tissues as well as in auto-immune inflammatory diseases.6,7 A patent has been filed for the use of A3AR and A3AR agonist as a diagnostic marker for therapeutic treatments of cancer and other diseases.

Gene ID: 140

UniProt: P0DMS8

Pathways: Hormone Transport, cAMP Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process