ADRA1B antibody (Extracellular)

Catalog No. ABIN7042897

Product Details anti-ADRA1B Antibody

Target: ADRA1B (Adrenergic, alpha-1B-, Receptor (ADRA1B))

Binding Specificity: AA 21-35, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADRA1B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (IF), Live Cell Imaging (LCI), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to α1B-Adrenergic Receptor

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: 15 amino acid residues identical, rat - 13,Mouse - 14

Characteristics: Anti-α1B-Adrenergic Receptor (extracellular) Antibody (ABIN7042897, ABIN7043914 and ABIN7043915)) is a highly specific antibody directed against an extracellular epitope of the human α1B-adrenoceptor. The antibody can be used in western blot, immunohistochemistry, live cell imaging and indirect flow cytometry applications. It has been designed to recognize α1B-adrenoceptor from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KNANFTGPNQTSSNS, corresponding to amino acid residues 21-35 of human alpha1B-adrenoceptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-AR018

Blocking Peptide: BLP-AR018

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ADRA1B

Target: ADRA1B (Adrenergic, alpha-1B-, Receptor (ADRA1B))

Alternative Name: ADRA1B (ADRA1B Products)

Background: Alpha-1B adrenoceptor, ADA1B, ADRA1B,Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions. Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Adrenoceptors are divided into three types: α1, α2 and β adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β3.1,2 They are expressed in nearly all peripheral tissues and in the central nervous system.1,2 All α1-adrenoceptors (α1-ARs) activate phospholipases C and A2.3 In addition to mobilizing intracellular calcium, the α1-ARs have also been shown to activate calcium influx via voltage-dependent and -independent calcium channels.4 α1B-Adrenergic receptor populations are greatest in the spleen, kidney, cerebellum, and fetal brain.5α1B-Adrenoceptor causes contraction of smooth muscle cells and thereby controls vascular tone, blood pressure, and accelerates the development of atherosclerosis.5

Alternative names: alpha1B-Adrenergic Receptor, Alpha-1B adrenoceptor, ADA1B, ADRA1B

Gene ID: 147

NCBI Accession: NM_000679

UniProt: P35368

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process