beta 2 Adrenergic Receptor antibody (Extracellular)

Catalog No. ABIN7042904

The Rabbit Polyclonal anti-beta 2 Adrenergic Receptor antibody is suitable to detect beta 2 Adrenergic Receptor in samples from Mouse. It has been validated for WB, IHC, FACS and LCI.

Quick Overview for beta 2 Adrenergic Receptor antibody (Extracellular) (ABIN7042904)

Target: beta 2 Adrenergic Receptor (ADRB2) (Adrenergic, beta-2-, Receptor, Surface (ADRB2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta 2 Adrenergic Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-beta 2 Adrenergic Receptor Antibody

Binding Specificity: AA 15-30, Extracellular

Purpose: A Rabbit Polyclonal Antibody to ?2-Adrenergic Receptor

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human - 15,16 amino acid residues identical, rat - 14

Characteristics: Anti-β2-Adrenergic Receptor (extracellular) Antibody (ABIN7042904, ABIN7043910 and ABIN7043911) is a highly specific antibody directed against an extracellular epitope of the mouse β2-adrenoceptor. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize β2AR from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)NGSRAPDHDVTQERDE, corresponding to amino acid residues 15-30 of mouse ADRB2 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7234676)

Blocking Peptide: (ABIN7234676)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for beta 2 Adrenergic Receptor

Target: beta 2 Adrenergic Receptor (ADRB2) (Adrenergic, beta-2-, Receptor, Surface (ADRB2))

Alternative Name: ADRB2

Background: Beta-2 adrenoceptor, Beta-2 adrenoreceptor, ADRB2, B2AR,Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions. Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Adrenoceptors are divided into three types: α1, α2 and β-adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β3.1,2 Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system.1,2 β2-Adrenoceptors are mainly postsynaptic receptors. They are expressed on a number of tissues including blood vessels, bronchi, gastrointestinal tract (GIT), skeletal muscle, liver and mast cell.3 Functional β2-adrenoceptors were also found on lymphocytes, macrophages and neutrophils.4,5 They are also the only β-adrenoceptor subtype expressed in keratinocytes, fibroblasts and melanocytes.3 Activation of β2-adrenoceptors results in vasodilation, relaxation of the GIT, inhibition of histamine release from mast cells and bronchodilation. Selective agonists of β2-adrenoceptors are used to treat asthma and other related bronchospastic conditions such as Chronic obstructive pulmonary disease (COPD).5

Alternative names: beta2-Adrenergic Receptor, Beta-2 adrenoceptor, Beta-2 adrenoreceptor, ADRB2, B2AR

Gene ID: 11555

NCBI Accession: NM_000024

UniProt: P18762

Pathways: cAMP Metabolic Process, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Brown Fat Cell Differentiation