ASIC3 antibody (Intracellular, N-Term)

Catalog No. ABIN7887114

The Guinea Pig Polyclonal anti-ASIC3 antibody is suitable to detect ASIC3 in samples from Rat. It has been validated for WB, IHC and IF.

Quick Overview for ASIC3 antibody (Intracellular, N-Term) (ABIN7887114)

Target: ASIC3 (ACCN3) (Amiloride-Sensitive Cation Channel 3 (ACCN3))

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This ASIC3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ASIC3 Antibody

Binding Specificity: AA 2-16, Intracellular, N-Term

Purpose: A Guinea Pig Polyclonal Antibody to ASIC3 Channel

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: 15 amino acid residues identical, human - 11,Mouse - 14

Characteristics: Guinea pig Anti-ASIC3 Antibody is directed against an epitope of rat ASIC3. Guinea pig Anti-ASIC3 Antibody raised in guinea pig can be used in western blot and immunohistochemistry applications. It has been designed to recognize ASIC3 from rat, human, and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-ASIC3 Antibody (ABIN7042917, ABIN7045243 and ABIN7045244) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Purification: Affinity purified on immobilized antigen.

Immunogen: KPRSGLEEAQRRQAS(C), corresponding to amino acid residues 2-16 of rat ASIC3

Isotype: IgG

Application Details

Application Notes:

WB: 1:1000

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: ICC|IHC

Negative Control: (ABIN7234708)

Blocking Peptide: (ABIN7234708)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for ASIC3

Target: ASIC3 (ACCN3) (Amiloride-Sensitive Cation Channel 3 (ACCN3))

Alternative Name: ASIC3

Background:

Synonyms: Acid-sensing ion channel 3, Amiloride-sensitive cation channel 3, ACCN3, DRASIC

Description: ASIC3 is a member of a family of Na+ channels that are activated by external protons. The family includes four additional members: ASIC1, ASIC2, ASIC4 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.The functional channel is composed of 4 subunits that can be assembled as homo- or heterotetramers with the other ASIC subunits.A drop in external pH opens the channel resulting in an inward cation current that is quickly inactivated even in the continuous presence of protons in the medium, although a small residual current may persist.Several lines of evidence indicate that ASIC3 may function as a pain sensor. First, it is specifically located in dorsal root ganglia (DRG) neurons and in nociceptors that are involved in pain detection and transmission. In addition, in conditions such as inflammation and cardiac ischemia that include tissue acidosis and thus pain, ASIC3 currents have been detected. Finally, ASIC3 deficient mice display altered sensitivity to high intensity pain produced by heat or acid.Interestingly, while in rats ASIC3 is expressed almost exclusively in DRG neurons, in humans its expression is more widespread suggesting a more extensive role in human nociception.

Gene ID: 286920

NCBI Accession: NM_004769

UniProt: O35240