ASIC3 antibody (Intracellular, N-Term)

Catalog No. ABIN7887112

The Rabbit Polyclonal anti-ASIC3 antibody (ABIN7887112) specifically detects ASIC3 in WB, IHC, IF and IC. The antibody is reactive with Rat samples.

Quick Overview for ASIC3 antibody (Intracellular, N-Term) (ABIN7887112)

Target: ASIC3 (ACCN3) (Amiloride-Sensitive Cation Channel 3 (ACCN3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASIC3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Grade: KO Validated

Product Details anti-ASIC3 Antibody

Binding Specificity: AA 2-16, Intracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to ASIC3 Channel

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: 15 amino acid residues identical, human - 11,Mouse - 14

Characteristics: Anti-ASIC3 Antibody (ABIN7042917, ABIN7045243 and ABIN7045244) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize ASIC3 from rat, human, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: KPRSGLEEAQRRQAS(C), corresponding to amino acid residues 2-16 of rat ASIC3

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:400

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: IHC

Negative Control: (ABIN7234708)

Blocking Peptide: (ABIN7234708)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for ASIC3

Target: ASIC3 (ACCN3) (Amiloride-Sensitive Cation Channel 3 (ACCN3))

Alternative Name: ASIC3

Background:

Synonyms: Acid-sensing ion channel 3, Amiloride-sensitive cation channel 3, ACCN3, DRASIC

Description: ASIC3 is a member of a family of Na+ channels that are activated by external protons. The family includes four additional members: ASIC1, ASIC2, ASIC4 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.The functional channel is composed of 4 subunits that can be assembled as homo- or heterotetramers with the other ASIC subunits.A drop in external pH opens the channel resulting in an inward cation current that is quickly inactivated even in the continuous presence of protons in the medium, although a small residual current may persist.Several lines of evidence indicate that ASIC3 may function as a pain sensor. First, it is specifically located in dorsal root ganglia (DRG) neurons and in nociceptors that are involved in pain detection and transmission. In addition, in conditions such as inflammation and cardiac ischemia that include tissue acidosis and thus pain, ASIC3 currents have been detected. Finally, ASIC3 deficient mice display altered sensitivity to high intensity pain produced by heat or acid.Interestingly, while in rats ASIC3 is expressed almost exclusively in DRG neurons, in humans its expression is more widespread suggesting a more extensive role in human nociception.

Gene ID: 286920

NCBI Accession: NM_004769

UniProt: O35240