MAS1 antibody (3rd Intracellular Loop)

Catalog No. ABIN7042920

The Rabbit Polyclonal anti-MAS1 antibody (ABIN7042920) specifically detects MAS1 in WB, IHC, IF and IC. The antibody is reactive with Rat samples.

Quick Overview for MAS1 antibody (3rd Intracellular Loop) (ABIN7042920)

Target: MAS1 (MAS1 Oncogene (MAS1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Grade: KO Validated

Product Details anti-MAS1 Antibody

Binding Specificity: 3rd Intracellular Loop, AA 212-224

Purpose: A Rabbit Polyclonal Antibody to Angiotensin-(1-7) Mas Receptor

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Angiotensin-(1-7) Mas Receptor Antibody (ABIN7042920, ABIN7043904 and ABIN7043905) is a highly specific antibody designed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize Ang-(1-7) Mas receptor from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KIRKNTWASHSSK, corresponding to amino acid residues 212-224 of the rat Angiotensin-(1-7) Mas receptor

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: IHC|ICC|IFC

Negative Control: (ABIN7234720)

Blocking Peptide: (ABIN7234720)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for MAS1

Target: MAS1 (MAS1 Oncogene (MAS1))

Alternative Name: MAS1

Background:

Synonyms: Proto-oncogene Mas, MAS1

Description: The Angiotensin-(1-7) Mas receptor is the recently identified receptor of the biologically active heptapeptide Angiotensin-(1-7).1Angiotensin (Ang)-(1-7) is a metabolite of the well known peptide hormone Angiotensin (Ang) II, a key component of the renin-angiotensin system (RAS) that has a central role in cardiovascular homeostasis.Considerably interest in Ang-(1-7) and its receptor aroused in the last few years since it became apparent that it can counterbalance most of Ang II effects. Thus Ang-(1-7) has vasodilator and hypotensive effects as well as antiarrhythmic and cardioprotective roles.2, 3The Ang-(1-7) Mas receptor belongs to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily and was originally described as a protooncogene.Signaling via the Ang-(1-7) Mas receptor is still poorly elucidated however, evidence indicates that the receptor is coupled to a Gq/11 protein that activates phospholipase C (PLC).2,3The Ang-(1-7) Mas receptor is expressed in several organs including heart, kidney, blood vessels, testis and brain. Studies with Ang-(1-7) Mas receptor knockout mice have demonstrated the key role of this receptor in cardiovascular regulation as well as in the regulation of learning and memory.2

Gene ID: 25153

NCBI Accession: NM_002377

UniProt: P12526

Pathways: ACE Inhibitor Pathway, Regulation of Carbohydrate Metabolic Process