MAS1 antibody (3rd Intracellular Loop)

Catalog No. ABIN7042921

This Guinea Pig Polyclonal antibody specifically detects MAS1 in WB. It exhibits reactivity toward Rat.

Quick Overview for MAS1 antibody (3rd Intracellular Loop) (ABIN7042921)

Target: MAS1 (MAS1 Oncogene (MAS1))

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This MAS1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAS1 Antibody

Binding Specificity: 3rd Intracellular Loop, AA 212-224

Purpose: A Guinea Pig Polyclonal Antibody to Angiotensin-(1-7) Mas Receptor

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Guinea pig Anti-Angiotensin-(1-7) Mas Receptor Antibody is directed against an epitope of the rat protein. Guinea pig Anti-Angiotensin-(1-7) Mas Receptor Antibody raised in guinea pig can be used in western blot analysis. It has been designed to recognize Ang-(1-7) Mas receptor from rat, mouse and human samples. The antigen used to immunize guinea pigs is the same as Anti-Angiotensin-(1-7) Mas Receptor Antibody (ABIN7042920, ABIN7043904 and ABIN7043905) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KIRKNTWASHSSK, corresponding to amino acid residues 212-224 of the rat Angiotensin-(1-7) Mas receptor

Isotype: IgG

Application Details

Application Notes:

WB: 1:600

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7234720)

Blocking Peptide: (ABIN7234720)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for MAS1

Target: MAS1 (MAS1 Oncogene (MAS1))

Alternative Name: MAS1

Background:

Synonyms: Proto-oncogene Mas, MAS1

Description: The Angiotensin-(1-7) Mas receptor is the recently identified receptor of the biologically active heptapeptide angiotensin-(1-7).1 Angiotensin (Ang)-(1-7) is a metabolite of the well known peptide hormone Angiotensin (Ang) II, a key component of the renin-angiotensin system (RAS) that has a central role in cardiovascular homeostasis. Considerably interest in Ang-(1-7) and its receptor aroused in the last few years since it became apparent that it can counterbalance most of Ang II effects. Thus Ang-(1-7) has vasodilator and hypotensive effects as well as antiarrhythmic and cardioprotective roles.2, 3 The Ang-(1-7) Mas receptor belongs to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily and was originally described as a protooncogene.Signaling via the Ang-(1-7) Mas receptor is still poorly elucidated however, evidence indicates that the receptor is coupled to a Gq/11 protein that activates phospholipase C (PLC).2,3 The Ang-(1-7) Mas receptor is expressed in several organs including heart, kidney, blood vessels, testis and brain. Studies with Ang-(1-7) Mas receptor knockout mice have demonstrated the key role of this receptor in cardiovascular regulation as well as in the regulation of learning and memory.2

Gene ID: 25153

NCBI Accession: NM_000789

UniProt: P12526

Pathways: ACE Inhibitor Pathway, Regulation of Carbohydrate Metabolic Process