AQP3 antibody (Intracellular)

Catalog No. ABIN7042937

The Rabbit Polyclonal anti-AQP3 antibody has been validated for WB, IHC, IF, IP and FACS. It is suitable to detect AQP3 in samples from Rat.

Quick Overview for AQP3 antibody (Intracellular) (ABIN7042937)

Target: AQP3 (Aquaporin 3 (Gill Blood Group) (AQP3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AQP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Grade: KO Validated

Product Details anti-AQP3 Antibody

Binding Specificity: AA 275-292, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Aquaporin 3 Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: 18 amino acid residues identical, sheep,human - 15,Mouse - 17

Characteristics: Anti-Aquaporin 3 Antibody (ABIN7042937, ABIN7045198 and ABIN7045199) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, and immunohistochemistry applications. It has been designed to recognize the AQP3 channel from rat, human, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)STEAENVKLAHMKHKEQI, corresponding to amino acid residues 275-292 of rat AQP3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC|IFC

Negative Control: (ABIN7234763)

Blocking Peptide: (ABIN7234763)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for AQP3

Target: AQP3 (Aquaporin 3 (Gill Blood Group) (AQP3))

Alternative Name: AQP3

Background: AQP3, Aquaglyceroporin-3, GIL, GLIP,Aquaporin 3 (AQP-3) belongs to a family of membrane proteins that allow the passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3 as well as AQP-7, AQP-9 and AQP-10.1 Little is known about the function of the two newest members, AQP-11 and AQP-12. The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.1AQP-3 is widely expressed in several organs with prominent expression found in the skin, colon, lung and kidney. Consistent with a central function of AQP-3 in skin, mice deficient in AQP-3 have reduced skin water content and elasticity compared with wild-type mice, as well as impaired wound healing and epidermal biosynthesis.2 Furthermore, AQP-3 deficient mice were found to be resistant to skin tumor development suggesting a role for this aquaporin in tumorigenesis.3

Alternative names: Aquaporin 3, AQP3, Aquaglyceroporin-3, GIL, GLIP

Gene ID: 65133

NCBI Accession: NM_004925

UniProt: P47862