Aquaporin 4 antibody (Intracellular) (Atto 594)

Catalog No. ABIN7042940

The Rabbit Polyclonal anti-Aquaporin 4 antibody has been validated for IHC and IF. It is suitable to detect Aquaporin 4 in samples from Rat.

Quick Overview for Aquaporin 4 antibody (Intracellular) (Atto 594) (ABIN7042940)

Target: Aquaporin 4 (AQP4)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aquaporin 4 antibody is conjugated to Atto 594

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Aquaporin 4 Antibody (Atto 594)

Binding Specificity: AA 300-314, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Aquaporin 4 Conjugated to the Fluorescent Dye ATTO-594

Specificity: Intracellular, C-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: human - 13,15 amino acid residues identical,Mouse - 14

Characteristics: Anti-Aquaporin 4 (AQP4) (300-314) Antibody (ABIN7042939, ABIN7045209 and ABIN7045210) is a highly specific antibody directed against an epitope of rat AQP4. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize the Aquaporin 4 channel from rat, mouse, and human samples. \nAnti-Aquaporin 4 (AQP4) (300-314)-ATTO Fluor-594 Antibody (ABIN7042940) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-Aquaporin 4 (AQP4) (300-314)-ATTO Fluor-594 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HVIDIDRGDEKKGKD, corresponding to amino acid residues 300-314 of rat AQP4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:80-1:100

Application Dilutions Western blot wb: N/A

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Aquaporin 4

Target: Aquaporin 4 (AQP4)

Alternative Name: AQP4

Background: WCH4, Mercurial-insensitive water channel,Aquaporin 4 (AQP-4) belongs to a family of membrane proteins that allow passage of water and certain solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).The aquaporins can be divided into two functional groups based on their permeability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP-4 together with AQP-1, AQP-2 and AQP-5 belong to the first group1. Little is known about the function of the two newest members, AQP-11 and AQP-12.The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores1.AQP-4 is the major membrane water channel in the central nervous system. The channel is expressed in astrocyte foot processes in direct contact with capillary vessels in the brain suggesting a role in water transport under normal and pathological conditions. Indeed, transgenic mice lacking AQP-4 have reduced brain swelling and improved neurological outcome following water intoxication and focal cerebral ischemia. In contrast, brain swelling and clinical outcome are worse in AQP-4-null mice in models of vasogenic (fluid leak) edema caused by freeze-injury and brain tumor, probably due to impaired AQP-4-dependent brain water clearance2.In addition, it has been recently shown that neuromyelitis optica (NMO), an inflammatory demyelinating disease that selectively affects optic nerves and spinal cord, is caused by the development of an autoantibody directed against AQP-43.

Alternative names: Aquaporin 4 (AQP4), WCH4, Mercurial-insensitive water channel

Gene ID: 25293

UniProt: P47863

Pathways: Sensory Perception of Sound