AQP9 antibody (Intracellular)

Catalog No. ABIN7042945

Product Details anti-AQP9 Antibody

Target: AQP9 (Aquaporin 9 (AQP9))

Binding Specificity: AA 4-19, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AQP9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to Aquaporin 9 Channel

Specificity: Intracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Unlikely to recognize human samples.

Predicted Reactivity: Mouse - 13,16 amino acid residues identical

Characteristics: Anti-Aquaporin 9 Antibody (ABIN7042945 and ABIN7045206)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize AQP9 from mouse and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EKDGAKKSLMQRLALK, corresponding to amino acid residues 4-19 of rat AQP9

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-QP009

Blocking Peptide: BLP-QP009

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for AQP9

Target: AQP9 (Aquaporin 9 (AQP9))

Alternative Name: AQP9 (AQP9 Products)

Background: AQP9, Aquaglyceroporin-9,Aquaporin 9 (AQP-9) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes.1 The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12.The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are permeated primarily by water (AQP-1, AQP-2, AQP4, AQP-5, AQP-6, AQP-8), and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3, AQP-7, AQP-9 and AQP-10.The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit contains four pores.2AQP-9 is expressed in several tissues including the brain, liver, testis and spleen. In the liver AQP-9 has a central role in glycerol metabolism as demonstrated in experiments using AQP-9 knockout mice: mice deficient in AQP-9 expression showed enhanced plasma levels of glycerol and triglycerides.3

Alternative names: Aquaporin 9, AQP9, Aquaglyceroporin-9

Gene ID: 65054

NCBI Accession: NM_020980

UniProt: P56627