N-Cadherin antibody (Intracellular)

Catalog No. ABIN7042976

The Rabbit Polyclonal anti-N-Cadherin antibody has been validated for WB and IHC. It is suitable to detect N-Cadherin in samples from Mouse.

Quick Overview for N-Cadherin antibody (Intracellular) (ABIN7042976)

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This N-Cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-N-Cadherin Antibody

Binding Specificity: AA 794-807, Intracellular

Purpose: A Rabbit Polyclonal Antibody to N-Cadherin

Specificity: Intracellular

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-N-Cadherin Antibody (ABIN7042976, ABIN7044708 and ABIN7044709) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize CDH2 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DTVEPDAIKPVGIR, corresponding to amino acid residues 794-807 of mouse CDH2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7234896)

Blocking Peptide: (ABIN7234896)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Alternative Name: CDH2

Background: Cadherin 2, Neural cadherin, CDH2, NCAD, CDHN, CD325, Calcium-dependent adhesion protein neuronal,Cadherins are transmembrane proteins responsible for cell adhesion. Cell adhesion is a crucial feature for the sustainability and maintenance of normal tissue function and three dimensional structure. Cadherins share an extracellular domain consisting of multiple repeats of cadherin-specific motif and are calcium dependent homotypic cell-to-cell adhesion molecules. They are localized mostly in specialized sites called adherence junctions1.N-cadherin consists of an amino-terminal external domain with five tandem repeats, a single transmembrane segment, and a cytoplasmic carboxy-terminal domain of approximately 150 amino acids. The intracellular domain of N-cadherin interacts with a group of proteins called catenins that are essential for cadherin-mediated cell adhesion. It is suggested that N-cadherin proteins align in a form of "zipper" when involved in cell adhesion. Cadherins on one cell surface form a series of rigid dimers that attach to equivalent dimers on the opposing cells and lateral motion of these complexes allows the cell junction site to "zip up"1.N-cadherin plays a role in tumor metastasis. Unlike E-cadherin that causes tumor metastasis when insufficiently, N-cadherin is involved in tumor metastasis when upregulated. N-cadherin stimulates migration, invasion and metastasis of squamous cell breast cancer and its effects are increased by FGF-2 suggesting that N-cadherin and FGFR-1 synergize and promote aggressive tumor behavior. This synergy also promotes neuronal growth1. Interestingly, low levels of N-cadherin are associated with a higher invasive capacity of astrocytic glioma by increasing tumor (and normal) cell migration speed and creating a less organized pattern of migration3.

Alternative names: N-Cadherin, Cadherin 2, Neural cadherin, CDH2, NCAD, CDHN, CD325, Calcium-dependent adhesion protein neuronal

Gene ID: 12558

NCBI Accession: NM_001792

UniProt: P15116

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane