CALCRL antibody (Extracellular, N-Term)

Catalog No. ABIN7042979

The Rabbit Polyclonal anti-CALCRL antibody is suitable to detect CALCRL in samples from Human. It has been validated for WB, IHC, FACS and LCI.

Quick Overview for CALCRL antibody (Extracellular, N-Term) (ABIN7042979)

Target: CALCRL (Calcitonin Receptor-Like (CALCRL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CALCRL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-CALCRL Antibody

Binding Specificity: AA 127-140, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Calcitonin Receptor-Like Receptor

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - 12,14 amino acid residues identical

Characteristics: Anti-CRLR/CALCRL (extracellular) Antibody (ABIN7042979, ABIN7044198 and ABIN7044199) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and indirect live cell flow cytometry. It has been designed to recognize CRLR from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: CNVNTHEKVKTALN, corresponding to amino acid residues 127 - 140 of human Calcitonin Receptor-Like Receptor 

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7234904)

Blocking Peptide: (ABIN7234904)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CALCRL

Target: CALCRL (Calcitonin Receptor-Like (CALCRL))

Alternative Name: CALCRL

Background:

Synonyms: Calcitonin Receptor-Like Receptor, Calcitonin Gene-Related Peptide Type 1 Receptor, CRL, CGRPR

Description: Calcitonin Receptor-Like Receptor 1 (CRLR or CGRPR) is a G protein-coupled receptor that binds the peptide hormone calcitonin and is involved in maintenance of calcium homeostasis, particularly with respect to bone formation and metabolism1.When engaged with its substrate, calcitonin gene related peptide (CGRP), CGRPR activates cAMP-dependent pkA and PI3 kinase, which eventually leads to complex inhibitory as well as facilitator actions on Neuronal Nicotinic Acetylcholine Receptor1.CGRP receptor is a heterodimer composed of two proteins: a 7-transmembrane domain type 2 G-protein coupled receptor called calcitonin-receptor-like receptor (CRLR), and receptor-activity-modifying protein 1 (RAMP1), a single-membrane-pass protein. RAMP1 serves as a transporter for CRLR to the cell surface and its absence leads to failure of binding with CRLR2.CGRP and CGRPR are ubiquitously expressed and their interaction is related to various diseases and health states such as migraines3 and Alzheimer's disease1.

Gene ID: 10203

NCBI Accession: NM_005795

UniProt: Q16602

Pathways: cAMP Metabolic Process