CABP1 antibody (Intracellular)

Catalog No. ABIN7042980

Product Details anti-CABP1 Antibody

Target: CABP1 (Calcium Binding Protein 1 (CABP1))

Binding Specificity: AA 256-270, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CABP1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to Calcium-Binding Protein 1

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-CaBP1 Antibody is directed against an epitope of rat Calcium-binding protein 1. Anti-CaBP1 Antibody (ABIN7042980, ABIN7044206 and ABIN7044207)) can be used in western blot analysis. It has been designed to recognize CaBP1 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)REAMRKLLGHQVGHR, corresponding to amino acid residues 256-270 of rat CaBP1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:600

Comment:

Negative Control: BLP-CS002

Blocking Peptide: BLP-CS002

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CABP1

Target: CABP1 (Calcium Binding Protein 1 (CABP1))

Alternative Name: CABP1 (CABP1 Products)

Background: Calcium-binding protein 1, Caldendrin, Calbrain,Neuronal Ca2+-binding proteins (CaBP1-5) are a subclass of the calmodulin (CaM) superfamily that regulates specific Ca2+ channel targets in the brain and retina. Multiple isoforms of CaBPs are localized in different neuronal cell types and perform specialized roles in sensory transduction and disease processes1.CaBP1-5 proteins have four EF hands that form pairs within the N lobe (EF1 and 2) and C lobe (EF3 and 4). The two lobes are structurally independent and connected by a flexible linker. Whereas all four EF hands bind Ca2+ in CaM, EF2 in CaBP1 does not bind Ca2+, and EF1 has reduced selectivity for Ca2+ over Mg2+. EF3 and EF4 in the C lobe of CaBP1 exhibit canonical Ca2+-induced conformational changes2. CaBP-target interactions induce functional changes distinct from those caused by CaM and may diversify neuronal responses to Ca2+ signals. CaBPs interact with and reshape the functional properties of certain voltage-gated Ca2+ channels (CaVs)3.CaBP1 is the best characterized family member and has been shown to regulate inositol 1,4,5-triphosphate receptors (IP3Rs), P/Q-type voltage-gated Ca2+ channels, L-type Ca2+ channels, and the transient receptor potential channel (TRPC5)4. CaBP1 regulates the currents of CaV1 and CaV2 channels in neurons, photoreceptors, and auditory hair cells. CaBP interactions with CaV1 channels may be required for hearing and vision, as mutations that disrupt these interactions cause blindness and deafness5.

Alternative names: CaBP1, Calcium-binding protein 1, Caldendrin, Calbrain

Gene ID: 171051

NCBI Accession: NM_001033677

UniProt: O88751

Pathways: Synaptic Membrane, Negative Regulation of Transporter Activity