CABP4 antibody (Intracellular)

Catalog No. ABIN7886268

The Rabbit Polyclonal anti-CABP4 antibody is suitable to detect CABP4 in samples from Mouse. It has been validated for WB.

Quick Overview for CABP4 antibody (Intracellular) (ABIN7886268)

Target: CABP4 (Calcium Binding Protein 4 (CABP4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CABP4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CABP4 Antibody

Binding Specificity: AA 105-120, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Calcium-Binding Protein 4

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Rat - identical, human - 14,15 amino acid residues identical

Characteristics: Anti-CaBP4 Antibody is directed against an epitope of mouse Calcium-binding protein 4. Anti-CaBP4 Antibody (ABIN7042982, ABIN7044210 and ABIN7044211) can be used in western blot analysis. It has been designed to recognize CaBP4 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RTYGPLLNRMFGKDR, corresponding to amino acid residues 105-120 of mouse CaBP4

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7234915)

Blocking Peptide: (ABIN7234915)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CABP4

Target: CABP4 (Calcium Binding Protein 4 (CABP4))

Alternative Name: CABP4

Background:

Synonyms: Calcium-binding protein 4

Description: Neuronal calcium-binding proteins (CaBP1-8) belong to a subclass of the calmodulin (CaM) superfamily and regulate in particular Ca2+ channel targets in the brain and retina1.CaBP4 contains two globular domains (N- and C-lobe) that each contains a pair of EF- hand motifs connected by a central linker. The second EF-hand in CaBP4 lacks conserved residues in the binding loop and is predicted to not bind Ca2+. CaBP4 interacts structurally with the C-terminal region of CaV1.4 and this Ca2+-dependent interaction has been implicated in the modulation of voltage-dependent CaV1.4 activation2.Multiple isoforms of CaBPs are localized in different neuronal cell types and perform specialized roles in signal transduction. The CaBP4 isoform regulates voltage-gated Ca2+ channels in retinal photoreceptor cells. In the eye, CaBP4 is localized at the photoreceptor synaptic terminals in rods and cones3. CaBP4 is essential for the development and maintenance of the photoreceptor synapse, likely through functional modulation of the photoreceptor Ca2+ channels, α1F L-type voltage- dependent calcium channels (CaV1.4), and neurotransmitter release4.Mutations in both CaV1.4 and CaBP4 have been identied in patients suffering from congenital stationary night blindness type 2 (CSNB2), indicating that defects in Ca2+-dependent regulation of CaV1.4 by CaBP4 are closely associated with CSNB2. Indeed, deletion of CaBP4 in the mouse leads to a CSNB2-like phenotype5.

Gene ID: 73660

NCBI Accession: NM_001300895

UniProt: Q8VHC5