CACNA1E antibody (Intracellular)

Catalog No. ABIN7043007

The Rabbit Polyclonal anti-CACNA1E antibody (ABIN7043007) specifically detects CACNA1E in WB, IHC, IF and IC. The antibody is reactive with Rat samples.

Quick Overview for CACNA1E antibody (Intracellular) (ABIN7043007)

Target: CACNA1E (Calcium Channel, Voltage-Dependent, R Type, alpha 1E Subunit (CACNA1E))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CACNA1E antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Grade: KO Validated

Product Details anti-CACNA1E Antibody

Binding Specificity: AA 892-907, Intracellular

Purpose: A Rabbit Polyclonal Antibody to CaV2.3 (CACNA1E) Channel

Specificity: Intracellular loop between domains II and III

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: rabbit - 12,16 amino acid residues identical, human - 11,Mouse - 14

Characteristics: Anti-CaV2.3 (CACNA1E) Antibody (ABIN7043007, ABIN7043957 and ABIN7043958) is directed against an epitope of the rat CaV2.3 channel. Anti-CaV2.3 (CACNA1E) Antibody (ABIN7043007, ABIN7043957 and ABIN7043958) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize CaV2.3 from human, mouse and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)SASQERSLDEGVSIDG, corresponding to amino acid residues 892-907 of rat CaV2.3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|Electrophysiology

Negative Control: (ABIN7234989)

Blocking Peptide: (ABIN7234989)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CACNA1E

Target: CACNA1E (Calcium Channel, Voltage-Dependent, R Type, alpha 1E Subunit (CACNA1E))

Alternative Name: CACNA1E

Background: CACH6, CACNL1A6, Voltage-dependent R-type calcium channel subunit α1E, BII, Brain calcium channel II, RBE2,Voltage-dependent Ca2+ channels (CaV channels) are pivotal players in many physiological roles such as secretion, contraction, migration and excitation.1The voltage-dependent Ca2+ channels are composed of several subunits, α1, β, α2δ and γ. CaV channels were originally divided into six physiological types: L-, N-, P-, Q-, R-, and T-type.1The CaV2.3 (formerly named α1E) protein, forms the R-type channels. R-type channels are highly sensitive to both SNX-482 (#RTS-500) and NiCl2. CaV2.3 channels were shown to mediate fast excitatory transmission in several synapses including the hippocampus.2CaV2.3 channels can be modulated by the muscarinic G-protein coupled receptors. In HEK-293 cells, expressing CaV2.3 channels, the R-type channel was shown to be both facilitated and inhibited by m1 and m2 muscarinic receptors. However, inhibition was much weaker than facilitation for the m1 receptor, while for m2 receptors, inhibition was much more pronounced than activation.3Facilitation and inhibition by the same receptors was probably exerted via different pathways as was indicated by their response to various inhibitors.3

Alternative names: CaV2.3 (CACNA1E), CACH6, CACNL1A6, Voltage-dependent R-type calcium channel subunit alpha1E, BII, Brain calcium channel II, RBE2

Gene ID: 54234

NCBI Accession: NM_000721

UniProt: Q07652

Pathways: Carbohydrate Homeostasis