CASR antibody (Extracellular) (FITC)

Catalog No. ABIN7043019

This anti-CASR antibody is a Rabbit Polyclonal antibody detecting CASR in FACS and LCI. Suitable for Human.

Quick Overview for CASR antibody (Extracellular) (FITC) (ABIN7043019)

Target: CASR (Calcium-Sensing Receptor (CASR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CASR antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-CASR Antibody (FITC)

Binding Specificity: AA 216-229, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Calcium Sensing Receptor Conjugated to the Fluorescent Dye FITC

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse,bovine,dog - identical

Characteristics: Anti-Calcium Sensing Receptor (extracellular) Antibody (ABIN7043018, ABIN7044161 and ABIN7044162) is a highly specific antibody directed against an epitope of the human CaSR. The antibody can be used in western blot, immunohistochemistry, indirect flow cytometry, and immunocytochemistry applications. It has been designed to recognize CaSR from human, rat, and mouse samples. \nAnti-Calcium Sensing Receptor (extracellular)-FITC Antibody (ABIN7043018, ABIN7044161 and ABIN7044162)-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry using live cells.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DDYGRPGIEKFREE, corresponding to amino acid residues 216-229 of human CaSR

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7235016)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CASR

Target: CASR (Calcium-Sensing Receptor (CASR))

Alternative Name: CASR

Background: CaSR, Extracellular calcium-sensing receptor, Parathyroid cell calcium-sensing receptor 1, PCaR1, GPRC2A,The calcium-sensing receptor (CaSR) is a class C G-protein-coupled receptor encoded by a single copy gene. CaSR plays a major role in the maintenance of a physiological serum ionized Ca2+ concentration by regulating the circulating levels of parathyroid hormone1.The N-terminus of the receptor is comprised of a signal peptide of 19 amino acids. This terminus directs the nascent CaSR polypeptide into the lumen of the endoplasmic reticulum (ER) and is then cleaved. The extracellular domain (ECD) of the mature protein has approximately 600 amino acids, comprised of a bi-lobed Venus-flytrap-like domain (VFT) connected by a cysteine-rich region and a short peptide linker to the seven transmembrane domain (TMD) with an intracellular C-terminal tail of 216 amino acids. CaSR functions as a homodimer with both covalent and noncovalent interactions between the VFT domains of each monomer. Ca2+ binds in the cleft between the two lobes of each VFT causing the lobes to close on one another and the VFT to rotate with transfer of a conformational change to the cysteine-rich region.In the parathyroid gland, up-regulation of CaSR by 1,25-dihydroxyvitamin D [1,25(OH)2D] makes the gland more responsive to extracellular Ca2+ and parathyroid hormone (PTH) secretion is reduced. While somatic mutations in CASR are rare, parathyroid glands from patients with primary or severe uremic secondary hyperparathyroidism have reduced CaSR expression2.

Alternative names: Calcium Sensing Receptor, CaSR, Extracellular calcium-sensing receptor, Parathyroid cell calcium-sensing receptor 1, PCaR1, GPRC2A

Gene ID: 846

NCBI Accession: NM_000388

UniProt: P41180

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis