CCR3 antibody (2nd Extracellular Loop)

Catalog No. ABIN7886247

The Rabbit Polyclonal anti-CCR3 antibody is suitable to detect CCR3 in samples from Human. It has been validated for WB, FACS and LCI.

Quick Overview for CCR3 antibody (2nd Extracellular Loop) (ABIN7886247)

Target: CCR3 (Chemokine (C-C Motif) Receptor 3 (CCR3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCR3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-CCR3 Antibody

Binding Specificity: 2nd Extracellular Loop, AA 183-197

Purpose: A Rabbit Polyclonal Antibody to CCR3 Chemokine Receptor

Cross-Reactivity (Details): Not recommended for use with rat and mouse samples.

Predicted Reactivity: Human only

Characteristics: Anti-Human CCR3 (extracellular) Antibody (ABIN7043033, ABIN7044172 and ABIN7044173) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize CCR3 from human samples only. The antibody will not recognize the receptor from mouse or rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: CSALYPEDTVYSWRH, corresponding to amino acid residues 183-197 of human CCR3

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5-5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CCR3

Target: CCR3 (Chemokine (C-C Motif) Receptor 3 (CCR3))

Alternative Name: CCR3

Background:

Synonyms: C-C chemokine receptor type 3, Eosinophil eotaxin receptor, CKR3, C-C CKR-3, CMKBR3, B-chemokine receptor, CD193 antigen

Description: CCR3 (C-C chemokine receptor type 3) is a seven transmembrane G-protein coupled receptor (GPCR) that acts as a key regulator in the homeostasis of the immune system. The receptor is responsible for recruiting eosinophils to local inflammatory tissues.CCR3 is the endogenous receptor for Eotaxin, C-C motif chemokine 11 (CCL11). The binding between CCR3 and CCL11 activates G-protein-dependent signaling pathways and thus enables the release of eosinophils progenitor cells and mature eosinophils to the affected tissue. Studies have shown that deficiency in CCR3 protein may reduce the binding of Eotaxin and inhibition of phosphatidylinositol-3-kinase (PI3K) signaling pathway activation and the inactivation of its subunit PI3Kγ1. Other CCR3 ligands include CCL5, CCL24, CCL26, CCL8, CCL7, and CCL121.CCR3 is linked to several type-I allergy diseases, including allergic rhinitis. In addition to CCR3 expression in eosinophils, CCR3 is also expressed in basophils, microglia, astrocytes and neurons in both the developing and adult brain1,2.The expression of CCR3 increases following human immunodeficiency virus infection and in reactive microglia and astrocytes around the amyloid deposits in Alzheimer's disease2.

Gene ID: 1232

NCBI Accession: NM_001164680

UniProt: P51677